downloadPdf_2There is only One NanoDrop™

Introducing NanoDrop™ One

Trusted by scientists worldwide, the Thermo Scientific™ NanoDrop™ range of low volume spectrophotometers have revolutionised the way nucleic acid and protein samples are analysed. Using patented sample-retention technology, they have enabled direct measurement of as little as 1μl of sample without the need for dilution. Intuitive software with pre-programmed methods have made the NanoDrop instruments invaluable tools in the lab. The next generation of NanoDrop One instruments take this even further and introduces Acclaro™ Sample Intelligence technology, allowing you to understand the quality of your samples before you use them for downstream processes.

Acclaro™ – Bringing you One step closer to success

  • Sophisticated spectral analysis algorithms identify contaminants and report a corrected concentration
  • An embedded sensor with digital image analysis monitors your samples, alerting to bubbles and other anomalies, thereby ensuring measurement integrity
  • Instant feedback about sample quality with on-demand technical support for guided troubleshooting

Streamline Your Workflow

  • ComOne-Pipet-handpact stand-alone instrument with integrated high-resolution colour touchscreen interface. Save time and valuable benchspace
  • Powerful auto-pathlength technology ensures accurate measurements for highly concentrated samples without dilution or prior knowledge of sample concentration
  • Patented sample retention technology allows direct low volume measurement without the need for expensive slides or special consumables
  • Ergonomic design with tilting and sliding screen to accommodate both left and right handed users. Auto measure feature for fast and convenient data acquisition
  • Versatile data management with the ability to print results via an optional thermal printer or transfer data via USB, Ethernet, Wi-Fi or an external computer. Provides flexibility for extended analysis and electronic archiving
  • Integrated cuvette reader in the OneC model provides flexibility for optical density and kinetic measurements (with stirring and temperature control) as well as increasing the dynamic range for dilute samples. For convenience the cuvette position can be used with the arm either up or down

Qualify Not Just Quantify Your Nucleic Acid Samples

containmentAnalysisAccurate concentration and purity evaluation of nucleic acid samples is critical to the success of downstream applications. Low template loads and the presence of residual contaminants can require lengthy troubleshooting and result in costly delays. Acclaro™ Sample Intelligence technology allows you to make properly informed decisions on your sample use and delivers unprecedented information on sample purity. Acclaro™ is fully integrated into the NanoDrop™ One software and gives the following information:

Acclaro™ Contaminant Alert

  • Acclaro™ uses sophisticated mathematical algorithms to analyse your sample data and notify when contaminants such as phenol and protein are present in your sample
  • Acclaro™ then provides a corrected analyte concentration

Acclaro™ Information Alert

Convenient and comprehensive troubleshooting / technical support tools at your fingertips.

  • Learn about possible causes of contaminants and recommended solutions
  • See how contaminants can alter your sample spectrum

proteinEvaluate Protein Samples Effectively

  • The NanoDrop™ One, as with other NanoDrop™ models provides accurate and reproducible quantification for protein samples. The NanoDrop™ One further simplifies the process by guiding you with a selection of protein applications, an intuitive Protein Editor and Acclaro™ contaminant information.
  • For direct A280 quantification of purified proteins, simply choose a suitable extinction coefficient from the pre-defined sample types or intuitively set up new sample types using the Protein Editor. Acclaro™ detects DNA and Phenol contamination ensuring better accuracy for your measurements
  • Quantify peptides or proteins that lack Tryptophan or Tyrosine residues by monitoring the peptide bond absorbance at 205nm
  • Verify protein labeling efficiency using the Proteins and Labels application
  • Select your favourite from a menu of pre-programmed colorimetric protein assays including Bradford, BCA, Lowry and Thermo Scientific™ Pierce™ 660nm protein assay

Evolve To Meet Your Needs

One-Pipet-cuvetteThe NanoDrop™ One instruments allow you to create and save custom analysis methods, run a kinetic assay or even generate a bacterial growth curve.

Custom Methods

  • Use pre-configured custom methods to analyse chlorophyll, nanoparticles, haemoglobin and more
  • Create and save new custom methods to analyse novel sample types
  • Use the UV-Vis application to monitor multiple wavelengths between 190-850nm


  • Use the pedestal or cuvette (OneC only) to monitor growth of microbial cultures. Enter the cell number conversion factor to automatically convert the 600nm value into the number of cells/ml


  • Intuitively create, edit and save custom kinetic methods using the cuvette option of the NanoDrop™ OneC spectrophotometer
Limit of Detection (dsDNA) Pedestal: 2.0 ng/μl Cuvette: 0.2 ng/μl
Limit of Detection (BSA) Pedestal: 0.06 mg/ml Cuvette: 0.006 mg/ml
Limit of Detection (IgG) Pedestal: 0.03 mg/ml Cuvette: 0.003 mg/ml
Maximum Concentration (dsDNA) Pedestal: 27,500 ng/μl
Maximum Concentration (BSA) Pedestal: 820 mg/ml
Maximum Concentration Pedestal: 400 mg/ml
Measurement and Data processing time 8 seconds
Measurement Repeatability Typical 0.002 A (1.0mm path) or 1% CV, whichever is greater
Wavelength Range 190-850 nm
Wavelength Accuracy ± 1 nm
Photometric Range (10 mm equivalent) Pedestal: 0-550 A Cuvette: 0-1.5 A
Accuracy 3% at 0.97 A, 302 nm
Resolution (Spectral Bandwidth) ≤1.8 nm (FWHM at Hg 254 nm)
Pathlength 0.03 – 1 mm auto ranging

Hardware and Software

Light Source Xenon Flash Lamp
Detector 2048 element CMOS linear image sensor
Dimensions (W x D x H) 20 x 25.4 x 32.3 cm
Weight 3.6 kg
Operating Voltage 12v DC
Power Consumption Operating 12-18 W Standby 5 W
Stirring (OneC cuvette only) 9 speeds
Temperature control (OneC cuvette only) 37°C
On-Board Control Operating system: Android™
CPU: Quad Core ARM®
Display: 7 inch, 1280 x 800 high-definition colour display
Touchscreen: Multipoint capacitive touch, compatible with lab gloves
Gesture recognition: Single point, single point
Internal Storage: 32 GB Flash Memory
Audio: Built-in speaker
Connectivity Three USB-A ports, Ethernet, Bluetooth and Wi-Fi
PC Software Requirements Windows® 8.1 and 10 (64 bit)
Accessory Support DYMO LabelWriter 450 printer, Bluetooth keyboard, mouse and barcode reader
Applications Support Nucleic Acid 260, A260/280, A260/230 and Labelled Nucleic Acids; Protein A280 and A205m Protein Pierce 660, Protein Bradford, Protein BCA, Protein Lowry  and Labelled Proteins; OD600, Kinetics, UV-Vis and Custom Methods
Language Support English,Chinese, French, German, Japanese, Korean, Polish and Spanish